Polymers in Regeneration

Methods

Beating area in the middle of murine embryoid body (EB) on polyetherurethane surface.

Beating area in the middle of murine embryoid body (EB) on polyetherurethane surface.

  • Cell isolation, cultivation and maintenance
  • Cell seeding approaches: direct seeding, centrifugation, injection, dynamic seeding with bioreactor etc.
  • Biocompatibility assay: cytotoxicity, apoptosis, senescence, proliferation, differentiation, angiogenesis, migration, gene and protein expression, cytoskeleton staining, morphology, etc.
  • Embryoid body formation of iPSCs via hanging drop or cell mass suspension culture
  • Quantitative PCR array
  • Bio-plex cytokine array
  • Microscopies: laser scanning microscopy, fluorescent microscopy, time-lapse microscopy etc.